Scientific-Technical Facilities

Chromatography

Facility Description

The IBVF High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) services are analytic services designed for the identification and quantification of different types of metabolites.

The chromatography services include system initialization and operation, the development of sample separation protocols, identification and quantification of the metabolites to be analyzed, the preparation of a results report (including calibration results), and the calculation of results for problematic samples.

Equipment

The services rely on Hitachi HPLC systems (DAD and fluorescence detector), Sciex QTrap 6500+ UPLC- Tandem Mass Spectrometry and two Shimadzu GC systems (FID and Mass Spectrometry Single Quad). Biorad medium-low pressure liquid chromatography (FPLC) equipment is also available for protein purification.

Operating Standards

Service technician is responsible for mounting the chromatography columns, programming the separation protocol, preparing the mobile phases and the sample vials, performing sample injections onto the column(s), and preparing the results report.

The equipment must be reserved sufficiently in advance by notifying a service technician so that the application(s) can be discussed and developed in the most timely manner.

The chromatography service supplies quality solvents and reactives necessary for the preparations of mobile phases and the correct development of applications, except for reactives that are rare or are infrequently used or that are needed in very small doses, in which case the user is responsible for providing them.

The user is responsible for extracting and derivitising (if necessary) the samples.

The FPLC equipment can be used by users with experience in its operation without the intervention of the service technician. Both your own columns and service columns can be used (check with the technician for available columns).

The results are delivered in PDF format or in an Excel spreadsheet. A copy of the results is temporarily stored on the equipment, to which the users have full access.

Rates

 

 


IBVF

IIQ-ICMS

CSIC-USE

AGE-OPIS-UNIV.

COMPANIES

HPLC(PDA,FL,IR)

3.39

3.68

3.97

10.52

11.52

GC (FID)

3.36

3.65

3.94

10.48

11.48

GC-FID Ethylene

2.29

2.48

2.68

5.82

6.38

GC-MS

3.36

3.68

3.94

10.48

11.48

FPLC*

4.70

5.10

5.51

22.98

25.17

FPLC + column*

18.57

20.15

21.87

41.06

44.97

LC-MS

5.34

5.79

6.26

49.57

54.30

 

Prices in €/sample.

Prices valid until March 2025.

 

Download rates as PDF

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