Salinity, nutrition and defence (SANUDE)
José M. Pardo, Francisco J. Quintero and Anna M. LindahlThe main losses in our agriculture are due to water deficit, salinity, and pathogen attack. Global warming is accentuating drought periods and altering the global distribution of pathogens. We aim to understand the molecular mechanisms by which plants perceive and integrate environmentally derived signals to specifically respond and acclimate to adverse and changing situations. The long-term goal of the Environmental Stress Signaling Group in Plants (SEAP) is to seek sustainable solutions to the climate crisis by identifying and studying genes and proteins that contribute to plant tolerance to environmental stress and resistance to pathogens, and use this knowledge to develop plants better adapted to adverse environmental conditions.
We are pursuing three research lines to study:
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- Response to salt stress, focusing on how sodium and potassium homeostasis are regulated during salt stress in Arabidopsis and rice plants.
- Regulation of potassium and nitrate mineral nutrition.
- Response to biotic stress using bacterial/tomato interaction as a model and identificacion of common intermediates in abiotic and biotic stresses.
In these research lines we characterize ion transporters in cell membranes, signal transduction mechanisms including calcium signaling and changes in cytosolic pH, and phosphorylation and redox modifications of proteins.
Our scientific-technical objectives are:
1. Improvement of sodium exclusion and redistribution in the plant by acting (targeting? (On) the SOS system, the main mechanism of sodium extrusion at the plasma membrane. We aim to understand how the SOS system interacts with regulators of flowering time under stress and with cytoskeleton dynamics. Enhanced halotolerance in rice plants.
2. Study of the regulation of potassium nutrition mediated by HAK-type transporters in roots. Structure-function relationships in HAK5 and its regulation by CIPK/CBL-type protein kinase modules.
3. Function and regulation of nitrate transporters of the NRT1/NPF family.
4. Optimization of the processes of vacuolar potassium uptake and storage mediated by NHX family ion exchangers, with emphasis on their protective capacity against water deficit and their regulatory function of stomatal activity and plant water balance.
5. Characterization of the CBL10/CIPK6 module and its phosphorylation targets in immunity in Solanaceae. Identification of common intermediates to simultaneously applied biotic and abiotic stress that could function as response integrators or switches.
Name | Surname | Category | Phones | |
---|---|---|---|---|
Raúl | Carranco Galán | Researcher Contract | ext. 139191 | |
Anna | De Luca | Researcher Contract | ext. 139191 | |
Olga | Del Pozo Cañas | CSIC Tenured Scientist | ext. 446018 | |
Nuria | Gavira Roldán | Laboratory Technician | ext. 139191 | |
Anna Marika | Lindahl | CSIC Tenured Scientist | ext. 489644 | |
Imelda | Mendoza Baisas | Specialized Higher Technician | ext. 489602 | |
José Manuel | Pardo Prieto | CSIC Research Professor | ext. 489602 | |
Fco. Javier | Quintero Toscano | CSIC Tenured Scientist | ext. 489646 | |
Natalia | Raddatz Cárdenas | Researcher Contract | ext. 139191 | |
Miriam | Romero Sánchez | Predoctoral Researcher | ext. 139191 |
- Daniel-Mozo M, Belén Rombolá-Caldentey, Mendoza I, Ragel P, De Luca A, Carranco R, Alcaide AM, Ausili A, Cubero B, Schumacher K, Quintero FJ, Albert A, Pardo JM. The vacuolar K+/H+ exchangers and calmodulin-like CML18 constitute a pH-sensing module that regulates K+ status in Arabidopsis. Science Advances. 2024; 10(46). doi: 10.1126/sciadv.adp7658
- Gámez-Arjona F, Park HJ, García E, Aman R, Villalta I, Raddatz N, Carranco R, Ali A, Ali Z, Zareen S, De Luca A, Leidi EO, Daniel-Mozo M, Xu ZY, Albert A, Kim WY, Pardo JM, Sánchez-Rodriguez C, Yun DJ, Quintero FJ. Inverse regulation of SOS1 and HKT1 protein localization and stability by SOS3/CBL4 in Arabidopsis thaliana. Proc Natl Acad Sci USA. 2024; 121(9):e2320657121. doi: 10.1073/pnas.2320657121.
- Chen C, He G, Li J, Perez-Hormaeche J, Becker T, Luo M, Wallrad L, Gao J, Li J, Pardo JM, Kudla J, Guo Y. A salt stress-activated GSO1-SOS2-SOS1 module protects the Arabidopsis root stem cell niche by enhancing sodium ion extrusion. EMBO J. 2023; 42(13):e113004. doi: 10.15252/embj.2022113004.
- Park HJ, Gámez-Arjona FM, Lindahl M, Aman R, Villalta I, Cha JY, Carranco R, Lim CJ, García E, Bressan RA, Lee SY, Valverde F, Sánchez-Rodríguez C, Pardo JM, Kim WY, Quintero FJ, Yun DJ. S-acylated and nucleus-localized SALT OVERLY SENSITIVE3/CALCINEURIN B-LIKE4 stabilizes GIGANTEA to regulate Arabidopsis flowering time under salt stress. Plant Cell. 2023; 35(1):298-317. doi: 10.1093/plcell/koac289.
- Steinhorst L, He G, Moore LK, Schültke S, Schmitz-Thom I, Cao Y, Hashimoto K, Andrés Z, Piepenburg K, Ragel P, Behera S, Almutairi BO, Batistič O, Wyganowski T, Köster P, Edel KH, Zhang C, Krebs M, Jiang C, Guo Y, Quintero FJ, Bock R, Kudla J. A Ca2+-sensor switch for tolerance to elevated salt stress in Arabidopsis. Developmental Cell. 2022; 57(17):2081-2094.e7. doi: 10.1016/j.devcel.2022.08.001.
- Cha JY, Kim J, Jeong SY, Shin GI, Ji MG, Hwang JW, Khaleda L, Liao X, Ahn G, Park HJ, Kim DY, Pardo JM, Lee SY, Yun DJ, Somers DE, Kim WY. The Na+/H+ antiporter SALT OVERLY SENSITIVE 1 regulates salt compensation of circadian rhythms by stabilizing GIGANTEA in Arabidopsis. Proc Natl Acad Sci USA. 2022; 119(33):e2207275119. doi: 10.1073/pnas.2207275119.
- Ródenas R, Ragel P, Nieves-Cordones M, Martínez-Martínez A, Amo J, Lara A, Martínez V, Quintero FJ, Pardo JM, Rubio F. Insights into the mechanisms of transport and regulation of the arabidopsis high-affinity K+ transporter HAK5. Plant Physiol. 2021; 185(4):1860-1874. doi: 10.1093/plphys/kiab028.
- Shen M, Lim CJ, Park J, Kim JE, Baek D, Nam J, Lee SY, Pardo JM, Kim WY, Mackey D, Yun DJ. HOS15 is a transcriptional corepressor of NPR1-mediated gene activation of plant immunity. Proc Natl Acad Sci USA. 2020; 117(48):30805-30815. doi: 10.1073/pnas.2016049117.
- Gradogna A, Scholz-Starke J, Pardo JM, Carpaneto A. Beyond the patch-clamp resolution: functional activity of nonelectrogenic vacuolar NHX proton/potassium antiporters and inhibition by phosphoinositides. New Phytol. 2021; 229(5):3026-3036. doi: 10.1111/nph.17021.
- Ali A, Pardo JM, Yun DJ. ABAting the Response: A Novel ABA Signal Terminator that Disrupts the Hormone Co-receptor Complex. Mol Plant. 2020; 13(9):1241-1243. doi: 10.1016/j.molp.2020.07.017.