M. Isabel Muro Pastor

Group: Gene expression and metabolic regulation in cyanobacteria

Position: Científica Titular CSIC/Tenured Scientist

Contact:

Laboratory: LS101

Office: DS104

Phone: 954489598 ext. 446098

email:

Formation

Degree on Biological Sciences, University of Seville, 1988

PhD on Biological Sciences, University of Seville, 1993

Postdoctoral Researcher, University of Paris-Sud, Orsay (France), 1994-1997

Postdoctoral contract UE 1998 IBVF

Postdoctoral contract University of Seville 1999-2001 IBVF

Ramón y Cajal contract 2001-2005 IBVF

Research Interests

My research focuses on the study of the regulation of gene expression in model cyanobacteria (Synechocystis sp. PCC 6803 and Nostoc sp. PCC 7120). More specifically, in analyzing the adaptation of these organisms to environmental conditions, especially aspects related to carbon and nitrogen metabolism. We are studying different mutants affected by glycogen metabolism or specific regulators of carbon flow. Based on basic knowledge we explore possible biotechnological applications of these mutants.

Project PID2022-138317NB-I00 (MCIN/AEI/10.13039/501100011033, FEDER, UE), 2023-2026

Publications

Domínguez-Lobo MT, Roldán M, Gutiérrez-Diánez AM, Florencio FJ, Muro-Pastor MI. Double blocking of carbon metabolism causes a large increase of Calvin-Benson cycle compounds in cyanobacteria. Plant Physiol. 2024 Feb 20:kiae083. doi: 10.1093/plphys/kiae083.

Bolay P, Rozbeh R, Muro-Pastor MI, Timm S, Hagemann M, Florencio FJ, Forchhammer K, Klähn S. The Novel P_II-Interacting Protein PirA Controls Flux into the Cyanobacterial Ornithine-Ammonia Cycle. mBio. 2021 Mar 23;12(2):e00229-21. doi: 10.1128/mBio.00229-21. PMID: 33758091; PMCID: PMC8092223.

Muro-Pastor MI, Cutillas-Farray Á, Pérez-Rodríguez L, Pérez-Saavedra J, Vega-de Armas A, Paredes A, Robles-Rengel R, Florencio FJ. CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria. Plant Physiol. 2020 Dec;184(4):1792-1810. doi: 10.1104/pp.20.00802. Epub 2020 Sep 8. PMID: 32900980; PMCID: PMC7723081.

Robles-Rengel R, Florencio FJ, Muro-Pastor MI. Redox interference in nitrogen status via oxidative stress is mediated by 2-oxoglutarate in cyanobacteria. New Phytol. 2019 Oct;224(1):216-228. doi: 10.1111/nph.15979. Epub 2019 Jul 2. PMID: 31168850.

Giner-Lamia J, Robles-Rengel R, Hernández-Prieto MA, Muro-Pastor MI, Florencio FJ, Futschik ME. Identification of the direct regulon of NtcA during early acclimation to nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803. Nucleic Acids Res. 2017 Nov 16;45(20):11800-11820. doi: 10.1093/nar/gkx860. PMID: 29036481; PMCID: PMC5714215.